Sequential electrogram collection using multipolar catheters (Pantaray, HD Grid, etc.) is increasingly used in persistent AF (persAF) mapping. How-ever, it is unknow whether sequential mapping is suitable for dominant fre-quency (DF) mapping, as DF tends to be spatiotemporally unstable. We aim to model and compare simultaneously- and sequentially- collected EGMs for DF mapping. 10 persAF patients undergoing left atrial (LA) ablation were enrolled. 2048-channel virtual EGMs (EnSite Array, Abbott; 5 mins) were analysed. After QRST subtraction, fast Fourier transform was used to estimate DF with 4-s sliding windows (2-s overlap). LA meshes were automatically segmented into 20 random-selected captures (Fig. A) to model multipolar catheter cap-tures. Sequential maps were generated altering time delay (gap) between cap-tures and compared with the simultaneous map. Correlation coefficients (CCs) and absolute difference between maps were calculated (Fig C i-ii). Av-erage DF map over 5 mins were generated as ‘gold standard‘(Fig. D) , aver-age DF map using shorter time duration ranging from 0 to 5 mins for all pa-tients were compared with the ‘gold standard‘ map (Fig. C iii). Similarity between simultaneous and sequential maps was low (CC: 0.13 ± 0.12) with significant differences between capture delays (p<0.05, Fig. C i). A considerable DF difference at each node (0.61 ± 0.22 Hz) was found (Fig. C ii). As expected, the CC was correlated with increased time for DF map calculation, while DF difference were inverse-correlated (Fig. C iii). Using data for 84 s, CC of 0.91 ±0.06 and DF error of 0.065±0.023 Hz were achieved compared with full-length data (5 mins). This was confirmed visually in Fig. D with fully reproducible 84-s maps. Sequentially collected DF maps with short duration (4 seconds) captures generate distinct results from simultaneously collected maps. A duration of 84 seconds per capture is required to achieve reproducible mapping.