The aim of this study was to assess the shape and deformation of the left ventricle (LV) throughout the cardiac cycle in patients with chronic heart failure (CHF) using geometric morphometrics. Geometric morphometrics is an approach to investigate shape features that are understudied in relation to heart problems. Based on 2D echocardiographic images, characteristics of the shape and deformation of the LV during the cardiac cycle were obtained in patients with CHF referred for cardiac resynchronization therapy (CRT). The characteristics were compared in patients who responded or did not respond to CRT. Prognostic features of LV deformation in end-systole and diastole were identified that discriminated responders from non-responders to CRT with 88.5% accuracy.