Bernardi et al. (2009) demonstrated that autonomic variables can synchronise with musical structure, showing significant entrainment effects. This study aims to replicate their findings using current music information research and time-frequency analysis methods, while evaluating additional physiological signal interactions. Continuous blood pressure (BP), ECG, and respiration were recorded from 24 participants (13 women, mean age 23.5 [95% CI: 22.2–24.8]), including 12 choristers and 12 non-choristers, whilst they listened to excerpts from Verdi's La Traviata and Nabucco, music tracks inducing the most prominent effects in the original study. Tempo, loudness, and music envelope features were extracted from beat-synchronised audio waveforms. Time-frequency coherence (TFC) was computed using the smoothed pseudo-Wigner-Ville distribution across all signal pairs. Group comparisons revealed no significant differences between choristers and non-choristers, except for elevated coherence in respiration–BP waveform pairs during Nabucco among choristers. Significant coherence was observed between Nabucco's musical features (tempo, loudness) and both diastolic and systolic BP, with the highest contrast against surrogate data (P < 0.0005). Additionally, coherence between physiological signals (e.g., RR–diastolic BP, respiration–BP waveform) was significantly higher during Nabucco compared to silence (P < 0.05). Visual inspection showed a strong match between Nabucco's loudness and BP waveform envelopes. These findings validate Bernardi et al.'s results and extend them by demonstrating music-induced modulation of inter-physiological coherence, highlighting Nabucco's distinctive entrainment effects.